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Cattle PDGFBB(Platelet Derived Growth Factor BB) ELISA Kit
ELK0727
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48 T $320.00
96 T $458.00
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Overview

Product name: Cattle PDGFBB(Platelet Derived Growth Factor BB) ELISA Kit
Reactivity: Cattle
Alternative Names: PDGF-BB
Assay Type: Sandwich
Sensitivity: 16.81 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PDGFBB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PDGFBB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PDGFBB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PDGFBB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 1.972 1.886
1500.00 1.500 1.414
750.00 1.189 1.103
375.00 0.765 0.679
187.50 0.566 0.480
93.75 0.285 0.199
46.88 0.114 0.028
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PDGFBB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFBB in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFBB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-97% 86-92% 89-103% 89-98%
EDTA plasma(n=5) 89-97% 85-97% 87-96% 85-96%
Heparin plasma(n=5) 95-103% 89-101% 87-98% 85-92%