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Rat MT(Melatonin) ELISA Kit
ELK0721
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Rat MT(Melatonin) ELISA Kit
Reactivity: Rat
Alternative Names: N-Acetyl-5-Methoxytryptamine
Assay Type: Competitive Inhibition
Sensitivity: 2.78 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 2.5h
Research Area: Endocrinology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 0.217
250.00 0.479
125.00 0.793
62.50 0.997
31.25 1.236
15.63 1.592
7.82 1.813
0.00 2.226

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MT and the recovery rates were calculated by comparing the measured value to the expected amount of MT in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 88-99% 93%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 87-96% 82-93% 89-97%
EDTA plasma(n=5) 85-93% 98-105% 87-98% 95-102%
Heparin plasma(n=5) 85-94% 79-96% 92-105% 91-99%