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EasyStep Human MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
ELK071ES
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48 T $412.00
96 T $588.00
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Overview

Product name: EasyStep Human MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
Assay Type: Sandwich
Sensitivity: 0.57 pg/mL
Standard: 500 pg/mL
Detection Range: 7.81-500 pg/mL
Sample Type: Serum, plasma, cell culture supernatants
Assay Length: 1.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1, and the Human MCP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MCP1. After TMB substrate solution is added, only those wells that contain Human MCP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.168 2.067
250.00 1.790 1.689
125.00 1.114 1.013
62.50 0.763 0.662
31.25 0.542 0.441
15.63 0.315 0.214
7.82 0.138 0.037
0.00 0.101 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MCP1 and the recovery rates were calculated by comparing the measured value to the expected amount of MCP1 in samples.
Matrix Recovery range Average
serum(n=5) 89-101% 95%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 80-94% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 85-96% 93-99% 87-101%
EDTA plasma(n=5) 86-95% 93-103% 87-98% 85-96%
Heparin plasma(n=5) 93-102% 87-96% 85-98% 91-103%

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