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EasyStep Human IFNg(Interferon Gamma) ELISA Kit
ELK067ES
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48 T $320.00
96 T $458.00
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Overview

Product name: EasyStep Human IFNg(Interferon Gamma) ELISA Kit
Reactivity: Human
Alternative Names: IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Assay Type: Sandwich
Sensitivity: 15 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, cell
Assay Length: 1.5h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.038 1.930
500.00 1.521 1.413
250.00 1.186 1.078
125.00 0.892 0.784
62.50 0.447 0.339
31.25 0.481 0.373
15.63 0.226 0.118
0.00 0.108 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 79-96% 93-106% 87-98%
EDTA plasma(n=5) 86-93% 93-101% 85-98% 90-101%
Heparin plasma(n=5) 78-92% 79-93% 94-106% 83-97%

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