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EasyStep Human IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit
ELK062ES
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64 T $412.00
112 T $588.00
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Overview

Product name: EasyStep Human IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit
Reactivity: Human
Alternative Names: FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor
Assay Type: Sandwich
Sensitivity: 1.4 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP7, and the Human IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP7. After TMB substrate solution is added, only those wells that contain Human IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.266 2.179
100.00 1.611 1.524
50.00 1.142 1.055
25.00 0.864 0.777
12.50 0.508 0.421
6.25 0.345 0.258
3.13 0.234 0.147
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IGFBP7 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP7 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 78-93% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 83-96% 78-98% 90-99%
EDTA plasma(n=5) 98-103% 86-92% 87-93% 93-102%
Heparin plasma(n=5) 89-97% 85-97% 87-96% 85-96%

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