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Human Anti-p62(Ubiquitin Binding Protein P62 Antibody) ELISA Kit
ELK0521
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-p62(Ubiquitin Binding Protein P62 Antibody) ELISA Kit
Reactivity: Human
Alternative Names: anti-p62; anyi-SQSTM1
Assay Type: Sandwich
Sensitivity: 0.049 ng/mL
Standard: 8 ng/mL
Detection Range: 0.13-8 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-p62. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-p62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-p62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-p62 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
8.00 2.136 2.047
4.00 1.528 1.439
2.00 1.107 1.018
1.00 0.827 0.738
0.50 0.521 0.432
0.25 0.373 0.284
0.13 0.206 0.117
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-p62 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-p62 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-p62 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 84-99% 85-97% 88-102%
EDTA plasma(n=5) 85-101% 93-104% 87-103% 91-102%
Heparin plasma(n=5) 83-97% 92-103% 87-98% 93-101%