Products

EasyStep Mouse E2(Estradiol) ELISA Kit
ELK021ES
Size： Price：
48 T $412.00
96 T $588.00
Download ① Send email to us

OverviewPublications

Product name: EasyStep Mouse E2(Estradiol) ELISA Kit
Reactivity: Mouse
Alternative Names: 17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type: Competitive Inhibition
Sensitivity: 25 pg/mL
Standard: 1600 pg/mL
Detection Range: 25-1600 pg/mL
Sample Type: Serum
Assay Length: 1.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2 antigen, and the Mouse E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1600.00 0.197
800.00 0.385
400.00 0.611
200.00 0.812
100.00 1.157
50.00 1.583
25.00 1.796
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 78-95% 86%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 87-96% 82-95% 92-105%
EDTA plasma(n=5) 90-99% 95-106% 87-101% 82-97%
Heparin plasma(n=5) 87-95% 85-97% 89-101% 85-97%

Product Components