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Human KL-6(Krebs Von den Lungen-6) ELISA Kit
ELK0179
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48 T $320.00
96 T $458.00
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Overview

Product name: Human KL-6(Krebs Von den Lungen-6) ELISA Kit
Reactivity: Human
Alternative Names: KL-6
Assay Type: Sandwich
Sensitivity: 14.21 U/mL
Standard: 2000 U/mL
Detection Range: 31.25-2000 U/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Interstitial lung disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
2000.00 1.954 1.856
1000.00 1.502 1.404
500.00 1.175 1.077
250.00 0.795 0.697
125.00 0.521 0.423
62.50 0.381 0.283
31.25 0.229 0.131
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
Matrix Recovery range Average
serum(n=5) 80-93% 86%
EDTA plasma(n=5) 94-105% 98%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 79-92% 88-97% 85-97%
EDTA plasma(n=5) 92-101% 82-98% 89-97% 85-93%
Heparin plasma(n=5) 86-101% 86-101% 87-96% 92-105%