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Rat KL-6(Krebs Von den Lungen-6) ELISA Kit
ELK0178
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat KL-6(Krebs Von den Lungen-6) ELISA Kit
Reactivity: Rat
Alternative Names: KL-6
Assay Type: Sandwich
Sensitivity: 13.44 U/mL
Standard: 2000 U/mL
Detection Range: 31.25-2000 U/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Interstitial lung disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
2000.00 2.138 2.034
1000.00 1.613 1.509
500.00 1.116 1.012
250.00 0.817 0.713
125.00 0.553 0.449
62.50 0.347 0.243
31.25 0.187 0.083
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
Matrix Recovery range Average
serum(n=5) 92-106% 99%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-103% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-93% 96-103% 97-105% 89-97%
EDTA plasma(n=5) 85-96% 87-101% 89-97% 85-94%
Heparin plasma(n=5) 86-99% 93-107% 85-94% 87-101%