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Rat ACV-A(Activin A) ELISA Kit
ELK0175
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat ACV-A(Activin A) ELISA Kit
Reactivity: Rat
Alternative Names: ACVA; Activin Beta A Beta A Homodimer
Assay Type: Sandwich
Sensitivity: 4.57 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.095 1.997
500.00 1.628 1.530
250.00 1.118 1.020
125.00 0.782 0.684
62.50 0.576 0.478
31.25 0.368 0.270
15.63 0.203 0.105
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ACV-A and the recovery rates were calculated by comparing the measured value to the expected amount of ACV-A in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACV-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-103% 81-92% 78-87% 91-99%
EDTA plasma(n=5) 86-98% 91-103% 85-97% 87-96%
Heparin plasma(n=5) 87-95% 85-97% 89-101% 85-97%