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Human ACV-A(Activin A) ELISA Kit
ELK0174
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human ACV-A(Activin A) ELISA Kit
Reactivity: Human
Alternative Names: ACVA; Activin Beta A Beta A Homodimer
Assay Type: Sandwich
Sensitivity: 4.71 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.274 2.169
500.00 1.527 1.422
250.00 1.034 0.929
125.00 0.841 0.736
62.50 0.521 0.416
31.25 0.364 0.259
15.63 0.146 0.041
0.00 0.105 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ACV-A and the recovery rates were calculated by comparing the measured value to the expected amount of ACV-A in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACV-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 86-95% 90-103% 87-90%
EDTA plasma(n=5) 92-102% 81-93% 86-98% 91-103%
Heparin plasma(n=5) 85-97% 87-96% 85-96% 85-95%