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8-iso-PGF2α(8-isoprostane) ELISA Kit
ELK0151
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: 8-iso-PGF2α(8-isoprostane) ELISA Kit
Reactivity: General
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Assay Type: Competitive Inhibition
Sensitivity: 4.47 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.197
500.00 0.385
250.00 0.611
125.00 0.812
62.50 1.157
31.25 1.583
15.63 1.796
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-iso-PGF2α and the recovery rates were calculated by comparing the measured value to the expected amount of 8-iso-PGF2α in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-iso-PGF2α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 85-101% 86-98% 96-107%
EDTA plasma(n=5) 95-103% 99-106% 78-92% 83-97%
Heparin plasma(n=5) 87-96% 85-98% 89-102% 85-98%