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Human VAP-1(VascularAdhesion Protein 1) ELISA Kit
ELK0137
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human VAP-1(VascularAdhesion Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: AOC3; HPAO; SSAO; Amine oxidase; copper containing 3
Assay Type: Sandwich
Sensitivity: 12.1 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology;Signal Transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VAP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VAP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VAP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.379 2.298
1000.00 1.517 1.436
500.00 1.151 1.070
250.00 0.618 0.537
125.00 0.508 0.427
62.50 0.342 0.261
31.25 0.234 0.153
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VAP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of VAP-1 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VAP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-101% 86-97% 88-95%
EDTA plasma(n=5) 88-97% 90-99% 85-97% 79-101%
Heparin plasma(n=5) 78-94% 97-105% 88-101% 82-96%