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Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
ELK0117
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
Reactivity: Rat
Alternative Names: ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1
Assay Type: Sandwich
Sensitivity: 18.3 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SREBP-1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SREBP-1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SREBP-1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SREBP-1C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 2.168 2.080
2000.00 1.569 1.481
1000.00 1.217 1.129
500.00 0.937 0.849
250.00 0.566 0.478
125.00 0.315 0.227
62.50 0.175 0.087
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SREBP-1C and the recovery rates were calculated by comparing the measured value to the expected amount of SREBP-1C in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 97-107% 102%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SREBP-1C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-96% 85-99% 79-93% 89-97%
EDTA plasma(n=5) 88-103% 86-92% 91-99% 95-102%
Heparin plasma(n=5) 79-91% 87-96% 83-101% 82-95%