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Mouse Anti-KLH IgG(Keyhole Limpet Hemocyanin IgG) ELISA Kit
ELK0111
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse Anti-KLH IgG(Keyhole Limpet Hemocyanin IgG) ELISA Kit
Reactivity: Mouse
Alternative Names: Keyhole limpet IgG
Assay Type: Sandwich
Sensitivity: 0.67 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates
Assay Length: 3.5h
Research Area: Immunology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.052 1.949
50.00 1.509 1.406
25.00 1.285 1.182
12.50 0.765 0.662
6.25 0.493 0.390
3.13 0.312 0.209
1.57 0.186 0.083
0.00 0.103 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-KLH IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-KLH IgG in samples.
Matrix Recovery range Average
serum(n=5) 86-98% 92%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-KLH IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 83-103% 98-105% 92-101%
EDTA plasma(n=5) 88-97% 79-92% 85-93% 89-97%
Heparin plasma(n=5) 85-94% 97-103% 82-90% 92-101%