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Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit
ELK0096
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48 T $265.00
96 T $376.00
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Overview

Product name: Human CoII2-1(Degradation Product of Type-II Collagen) ELISA Kit
Reactivity: Human
Alternative Names: CoII2-1
Assay Type: Sandwich
Sensitivity: 18.2 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CoII2-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CoII2-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CoII2-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CoII2-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 2.219 2.138
1500.00 1.586 1.505
750.00 1.034 0.953
375.00 0.828 0.747
187.50 0.477 0.396
93.75 0.363 0.282
46.88 0.247 0.166
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CoII2-1 and the recovery rates were calculated by comparing the measured value to the expected amount of CoII2-1 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 78-96% 87%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CoII2-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-95% 93-103% 85-94% 87-101%
EDTA plasma(n=5) 79-93% 86-92% 84-98% 89-102%
Heparin plasma(n=5) 90-99% 87-103% 78-91% 83-97%