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Cattle Zn-MT(Zn-Metallothionein) ELISA Kit
ELK0090
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Cattle Zn-MT(Zn-Metallothionein) ELISA Kit
Reactivity: Cattle
Alternative Names: Zn-MT
Assay Type: Sandwich
Sensitivity: 0.51 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Zn-MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Zn-MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Zn-MT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Zn-MT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.172 2.069
50.00 1.657 1.554
25.00 1.251 1.148
12.50 0.831 0.728
6.25 0.427 0.324
3.13 0.372 0.269
1.57 0.262 0.159
0.00 0.103 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Zn-MT and the recovery rates were calculated by comparing the measured value to the expected amount of Zn-MT in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 80-93% 86%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Zn-MT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 91-105% 87-99% 99-105%
EDTA plasma(n=5) 88-95% 96-102% 89-103% 89-96%
Heparin plasma(n=5) 87-101% 87-96% 82-93% 89-97%