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Mouse NTS(Neurotensin) ELISA Kit
ELK0079
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48 T $265.00
96 T $376.00
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Overview

Product name: Mouse NTS(Neurotensin) ELISA Kit
Reactivity: Mouse
Alternative Names: NEUT; neuromedin N; NN; NT; NT/N; NTS1; NMN-125
Assay Type: Competitive Inhibition
Sensitivity: 12.2 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway
Uniprot ID: Q9D3P9
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NTS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NTS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NTS in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 0.183
1500.00 0.310
750.00 0.596
375.00 0.842
187.50 1.163
93.75 1.457
46.88 1.786
0.00 2.028

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NTS and the recovery rates were calculated by comparing the measured value to the expected amount of NTS in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NTS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 82-92% 86-94% 81-95%
EDTA plasma(n=5) 86-97% 88-95% 92-101% 87-98%
Heparin plasma(n=5) 85-92% 79-96% 92-101% 87-98%