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Human Gd-IgA1(Galactose-Deficient IgA1) ELISA Kit
ELK0072
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Gd-IgA1(Galactose-Deficient IgA1) ELISA Kit
Reactivity: Human
Alternative Names: Gd-IgA1
Assay Type: Sandwich
Sensitivity: 5.31 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Kidney biomarker;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Gd-IgA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Gd-IgA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Gd-IgA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Gd-IgA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 1.941 1.859
500.00 1.472 1.390
250.00 1.227 1.145
125.00 0.872 0.790
62.50 0.547 0.465
31.25 0.384 0.302
15.63 0.221 0.139
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Gd-IgA1 and the recovery rates were calculated by comparing the measured value to the expected amount of Gd-IgA1 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 92-105% 98%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gd-IgA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-98% 85-92% 83-96% 93-102%
EDTA plasma(n=5) 92-105% 85-101% 85-94% 87-96%
Heparin plasma(n=5) 86-97% 88-95% 92-101% 87-98%