Products

Human NP-B(Neuropeptide B) ELISA Kit
ELK0059
Size： Price：
48 T $320.00
96 T $458.00
Manual MSDS Send email to us

Overview

Product name: Human NP-B(Neuropeptide B) ELISA Kit
Reactivity: Human
Alternative Names: L7; PPNPB; PPL7; NPB
Assay Type: Sandwich
Sensitivity: 26.3 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;Hormone metabolism;
Uniprot ID: Q8NG41
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NP-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NP-B in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.172 2.076
2500.00 1.643 1.547
1250.00 1.174 1.078
625.00 0.728 0.632
312.50 0.496 0.400
156.25 0.276 0.180
78.13 0.241 0.145
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NP-B and the recovery rates were calculated by comparing the measured value to the expected amount of NP-B in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NP-B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 93-106% 83-95% 88-103%
EDTA plasma(n=5) 87-95% 85-97% 89-101% 85-97%
Heparin plasma(n=5) 86-93% 91-105% 87-99% 99-105%