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DPPH (1,1 - Diphenyl - 2 - picrylhydrazyl radical) is 1,1 - diphenyl - 2 - picrylhydrazyl radical. It is widely used for the quantitative determination of the antioxidant capacity of biological samples and foods. This method is based on the characteristic that DPPH radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When a free radical scavenger is present, due to the pairing with its single electron, the absorption gradually disappears, and the lighter the color, that is, the lower the A value. Then, the DPPH scavenging capacity in the sample can be quantitatively analyzed. |
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