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EasyStep Human HBP(Heparin-Binding Protein) ELISA Kit
ELK015ES
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48 T $412.00
96 T $588.00
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Overview

Product name: EasyStep Human HBP(Heparin-Binding Protein) ELISA Kit
Reactivity: Human
Alternative Names: AZU; AZAMP; AZU1; HBP; HUMAZUR; NAZC; CAP37; Cationic Antimicrobial Protein 37; Heparin-Binding Protein; Neutrophil Azurocidin
Assay Type: Sandwich
Sensitivity: 39 pg/mL
Standard: 2500 pg/mL
Detection Range: 39-2500 pg/mL
Sample Type: Serum
Assay Length: 1.5h
Research Area: Enzyme & Kinase;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBP, and the Human HBP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human HBP. After TMB substrate solution is added, only those wells that contain Human HBP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2500.00 2.126 2.022
1250.00 1.602 1.498
625.00 1.186 1.082
312.50 0.782 0.678
156.25 0.563 0.459
78.13 0.305 0.201
39.07 0.186 0.082
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HBP and the recovery rates were calculated by comparing the measured value to the expected amount of HBP in samples.
Matrix Recovery range Average
serum(n=5) 96-107% 102%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 85-98% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HBP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 81-96% 87-105% 83-92%
EDTA plasma(n=5) 88-101% 90-98% 92-106% 85-97%
Heparin plasma(n=5) 89-97% 85-97% 87-96% 85-96%

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