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Mycoplasma simple detection method
Extract the RNA of the cells to be tested by Trizol method (recommended to use TRIpure Total RNA Extraction Reagent Cat. No.: EP013), reverse transcribed into cDNA Detection primer PCR detection of Mycoplasma contamination of cells. Mycoplasma detection primers Mycoplasm
2022-06-23
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Proficiently manipulate the UniProt database
1,Introduction and use details of Uniprot protein database Uniprot database is the most widely resourced and most informative protein database, and the first choice for querying protein functions. The Uniprot database consists of three sub-databases, Swiss-Prot, TrEMBL and PIR-PSD. The data mainl
2022-06-09
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A brief introduction to the principle and detailed experimental steps of co-immunoprecipitation (Co-IP)
1.Principle Co-immunoprecipitation (Co-Immunoprecipitation) is a classic method for studying protein interactions based on the specific interaction between antibodies and antigens. It is an effective method to determine the physiological interaction of two proteins in intact cells. &
2022-06-08
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RNA Sample Preparation Guide
1、RNA SAMPLE yield and minimum input amount for various projects Total RNA yield statistics of each sample (for reference only)： Recommended starting amount for sequencing projects（the starting amount is for reference only,exosomes do not allow 18/s28s rRNA peaks）： Expression Gene C
2022-06-08
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What is ELISA--Principle and Classification
1. Basic Principles In 1971, Engvall and Perlmann published an article on enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of IgG, which made the enzyme-labeled antibody technology for antigen localization developed in 1966 to be used for the determination of trace
2022-05-23
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How to choose loading control antibodies
The loading control antibody can be used to evaluate the efficiency of Western Blot and compare the amount of protein loaded per well in the gel. Such controls can help identify differences in expression levels between samples, whether due to actual protein levels in cell lysates or differences
2022-05-19
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Calculation of ELISA results by two software
Method 1: Use OriginPro 2021 software to calculate the ELISA results of the competition kit Step 1: We take PG(Progesterone) ELISA Kit as an example Copy and paste the data of the standard curve into the following table. Press Ctrl+A to select all and then click in order:An
2022-05-11
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Westernblot experimental protein extraction
Adherent cell total protein extraction: Rinse the adherent cells 2-3 times with TBS buffer, blotting the remaining liquid as much as possible the last time. Add an appropriate volume of cell total protein extraction reagent (add protease inhibitor within minutes before use) and lyse in the c
2022-05-11