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EasyStep Human Cys-C(CystatinC) ELISA Kit
ELK005ES
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48 T $320.00
96 T $458.00
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Overview

Product name: EasyStep Human Cys-C(CystatinC) ELISA Kit
Reactivity: Human
Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type: Sandwich
Sensitivity: 25 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.1-5000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.781 2.716
2500.00 1.701 1.637
1250.00 0.906 0.842
625.00 0.508 0.443
312.50 0.319 0.254
156.25 0.177 0.113
78.13 0.120 0.056
0.00 0.064 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix Recovery range Average
serum(n=5) 88-99% 94%
EDTA plasma(n=5) 102-110% 104%
Heparin plasma(n=5) 98-104% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-106% 88-102% 85-101% 96-107%
EDTA plasma(n=5) 90-103% 94-108% 90-106% 98-105%
Heparin plasma(n=5) 91-100% 98-107% 92-108% 93-102%

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