|Product name:||CK16 Mouse mAb|
|Species reactivity:||Human, Rat, Mouse|
|Reactivity:||Human, Rat, Mouse|
|Alternative Names:||Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK|
|Recommended dilutions:||IHC 1:200|
|Storage:||PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.|
|Human Swiss-Prot No.:||P08779|
|Cellular localization:||Intermediate filament|
|Background:||Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.