CK16 Mouse mAb-ELK (Wuhan) Biotechnology CO.,Ltd.

Specification：	Price：
50 µl	$148.00
100 µl	$248.00

Product name:	CK16 Mouse mAb
Reactivity:	Human, Rat, Mouse
Alternative Names:	Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK
Source:	Mouse
Applications:	IHC
Species:	Human, Rat, Mouse
Species:	Human
Recommended dilutions:	IHC 1:200
Immunogen:	Synthetic Peptide
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/ml
Observed band:	51kDa
GeneID（Human）:	3868
Human Swiss-Prot No:	P08779
Cellular localization:	Intermediate filament
Background:	Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.