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CK16 Mouse mAb
EM1056
Specification: Price:
50 µl $148.00
100 µl $248.00

Overview

Product name: CK16 Mouse mAb
Species reactivity: Human, Rat, Mouse
Reactivity: Human, Rat, Mouse
Alternative Names: Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK
Source: Mouse
Applications : IHC
Species: Human
Recommended dilutions: IHC 1:200
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 51kDa
GeneID(Human): 3868
Human Swiss-Prot No.: P08779
Cellular localization: Intermediate filament
Background: Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.