|Product name:||GAPDH(HRP Conjugated) Mouse mAb|
|Species reactivity:||Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast|
|Reactivity:||Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast|
|Alternative Names:||G3PD, G3PDH, GAPD, Glyceraldehyde 3 phosphoate dehycdrogenase, MGC102544|
|Recommended dilutions:||WB 1:2,000-5,000 IHC 1:100|
|Storage:||PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.|
|Human Swiss-Prot No.:||P04406|
|Background:||Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.