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GAPDH(HRP Conjugated) Mouse mAb
EM1039
Specification: Price:
50 µl $148.00
100 µl $248.00

Overview

Product name: GAPDH(HRP Conjugated) Mouse mAb
Species reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast
Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast
Alternative Names: G3PD, G3PDH, GAPD, Glyceraldehyde 3 phosphoate dehycdrogenase, MGC102544
Source: Mouse
Applications : WB
Species: Human
Recommended dilutions: WB 1:2,000-5,000 IHC 1:100
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 37kDa
GeneID(Human): 2597
Human Swiss-Prot No.: P04406
Cellular localization: Cytoplasm,Cytoskeleton,Membrane,Nucleus
Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.