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β-actin(HRP Conjugated) Mouse mAb
EM1038
Specification: Price:
50 µl $148.00
100 µl $248.00

Overview

Product name: β-actin(HRP Conjugated) Mouse mAb
Species reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect
Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect
Alternative Names: actin antibody, b-actin antibody, beta-actin antibody
Source: Mouse
Applications : WB
Species: Human
Recommended dilutions: WB 1:2,000-5,000 IHC 1:100
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 43kDa
GeneID(Human): 60
Human Swiss-Prot No.: P60709
Cellular localization: Cytoplasm,Cytoskeleton
Background: β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.