|Product name:||Histone H3 Mouse mAb|
|Species reactivity:||Human, Mouse, Rat, Yeast|
|Reactivity:||Human, Mouse, Rat, Yeast|
|Alternative Names:||H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody|
|Applications :||WB, IHC, IF, IP|
|Recommended dilutions:||WB 1:2,000-5,000 IP 1:200 IF 1:200|
|Storage:||PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.|
|Human Swiss-Prot No.:||P68431|
|Background:||Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.