|Product name:||E1(Estrone) ELISA Kit|
|Assay Type:||Competitive Inhibition|
|Sample Type:||Serum, plasma and other biological fluids|
|Research Area:||Metabolic pathway;Endocrinology;Reproductive science;Genetic science;Hormone metabolism;|
|Test principle:||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrone(E1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrone(E1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrone(E1) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.