||This kit uses a unique buffer system that combines with traditional isopropanol precipitation to quickly obtain large amounts of high-purity plasmid DNA. The plasmid DNA extracted using this kit can be applied to various routine operations. Including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of a variety of cell experiments.It is recommended to use the amount of bacteria every time: the recommended amount of high-copy plasmid is 100 ml. the yield is generally about 200-1500ug the recommended amount of low-copy plasmid is 200 ml. and the yield is generally about 100-400ug.