| Product name: |
ATG5 (3C7) Mouse mAb |
| Reactivity: |
Human, Mouse, Rat |
| Alternative Names: |
APG5, APG5L, ASP, Autophagy protein 5 antibody |
| Source: |
Mouse |
| Applications: |
WB, IHC |
| Species: |
Human |
| Recommended dilutions: |
WB 1:5,000-10,000 IHC 1:100-200 |
| Immunogen: |
Recombinant Protein |
| Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: |
Monoclonal |
| Isotype: |
IgG1 |
| Concentration: |
1mg/ml |
| Observed band: |
55kDa |
| GeneID（Human）: |
9494 |
| Human Swiss-Prot No: |
Q9H1Y0 |
| Cellular localization: |
Cytoplasm, Membrane |
| Background: |
Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10. |
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Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200.
Western blot analysis of Hela Cell Lysate using ATG5 （EM1374）Mouse mAb diluted at 1:10000.