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Cattle RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit
ELK9153
Specification: Price:
48 T $412.00
96 T $588.00

Overview

Product name: Cattle RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit
Reactivity: Cattle
Alternative Names: CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5
Assay Type: Sandwich
Sensitivity: 1.31 ng/mL
Standard: 200 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 4.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted(RANTES). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted(RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulated On Activation In Normal T-Cell Expressed And Secreted(RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted(RANTES) in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.135 2.063
100.00 1.628 1.556
50.00 1.194 1.122
25.00 0.828 0.756
12.50 0.496 0.424
6.25 0.357 0.285
3.13 0.199 0.127
0.00 0.072 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant RANTES and the recovery rates were calculated by comparing the measured value to the expected amount of RANTES in samples.
Matrix Recovery range Average
serum(n=5) 89-107% 98%
EDTA plasma(n=5) 94-107% 100%
Heparin plasma(n=5) 81-93% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RANTES and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 81-94% 89-98% 90-99%
EDTA plasma(n=5) 95-106% 87-101% 86-93% 93-102%
Heparin plasma(n=5) 87-98% 83-97% 91-98% 83-101%