|Product name:||Human OxHDL(Oxidized high-density lipoprotein) ELISA Kit|
|Alternative Names:||Oxidized high-density lipoprotein|
|Sample type:||Serum, plasma, tissue homogenates and other biological fluids.|
|Research Area:||Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism|
|Test principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to OxHDL(Oxidized high-density lipoprotein). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to OxHDL(Oxidized high-density lipoprotein). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OxHDL(Oxidized high-density lipoprotein), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OxHDL(Oxidized high-density lipoprotein) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.