TRIpure Total RNA Extraction Reagent
Catalog No. : EP013
|Product name：||TRIpure Total RNA Extraction Reagent|
|Introduction：||"TRIpure Total RNA Extraction Reagent is a reagent for total RNA extraction from various animal and plant, bacterial tissues/cells. It has excellent cleavage ability to lyse cells and tissue samples in a short time, keeping RNA intact in the sample. Sexually, effectively inhibits the degradation of RNA. The sample can be fully cleaved in the reagent. After centrifugation with chloroform, the solution forms a supernatant layer, an intermediate layer and an organic layer (lower red layer). The RNA is distributed in the upper aqueous phase, and the supernatant layer is collected. Total RNA can be recovered by precipitation of propanol. The extracted total RNA is high in purity and contains no protein or genomic DNA. It can be directly used for Northern, dot hybridization, mRNA purification, in vitro translation, RT-PCR, poly(A)+ selection, RNase protection analysis, and construction of cDNA libraries. A variety of molecular biology experiments.
In addition, after removal of the aqueous layer, the DNA and protein in the sample can also be successively reduced by precipitation. Ethanol precipitation can precipitate the DNA of the intermediate layer, and isopropanol can be added to the organic layer to precipitate the protein.
This product is simple and fast to operate, and all operations can be completed in one hour. And a small amount of tissue (50-100mg) and cells (5 × 106) and a large number of tissues (≥ 1g) and cells (> 107) have a good lysis effect.
|Storage：||The kit can be stored for 12 months at room temperature (15–25 ° C) and at 2–8 ° C for longer periods of time.|