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Sheep IgE(Immunoglobulin E) ELISA Kit

Catalog No. : ELK8726

Specification Price
48T $412
96T $588

Overview

Product name: Sheep IgE(Immunoglobulin E) ELISA Kit
Alternative Names: IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
Reacted Species: Sheep
Assay Type: Sandwich
Sensitivity: 0.059 ug/mL
Standard: 10 ug/mL
Range: 0.16-10 ug/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 4.5h
Research Area: Infection immunity;Immune molecule;Hematology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin E(IgE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin E(IgE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin E(IgE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin E(IgE) in the samples is then determined by comparing the OD of the samples to the standard curve.