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Sheep IgG(Immunoglobulin G) ELISA Kit

Catalog No. : ELK8725

Specification Price
48T $370
96T $528

Overview

Product name: Sheep IgG(Immunoglobulin G) ELISA Kit
Alternative Names: Immunoglobulin G
Reacted Species: Sheep
Assay Type: Competitive Inhibition
Sensitivity: 0.55 μg/mL
Standard: 100 μg/mL
Range: 1.57-100 μg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 2h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G(IgG) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G(IgG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G(IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.