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Human PAX6(Paired Box Gene 6) ELISA Kit

Catalog No. : ELK8427

Specification Price
48T $320
96T $458

Overview

Product name: Human PAX6(Paired Box Gene 6) ELISA Kit
Alternative Names: AN; AN2; MGDA; WAGR; Oculorhombin; Aniridia,Keratitis; Aniridia type II protein
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 0.103 ng/mL
Standard: 20 ng/mL
Range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 4.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paired Box Gene 6(PAX6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paired Box Gene 6(PAX6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paired Box Gene 6(PAX6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paired Box Gene 6(PAX6) in the samples is then determined by comparing the OD of the samples to the standard curve.