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Rat RTN4(Reticulon 4) ELISA Kit

Catalog No. : ELK8424

Specification Price
48T $320
96T $458

Overview

Product name: Rat RTN4(Reticulon 4) ELISA Kit
Alternative Names: NSP; ASY; NOGO; NSP-CL; RTN-X; Reticulon-5; Foocen; Neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog
Reacted Species: Rat
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 4.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reticulon 4(RTN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reticulon 4(RTN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Reticulon 4(RTN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reticulon 4(RTN4) in the samples is then determined by comparing the OD of the samples to the standard curve.