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Human SDHA(Succinate Dehydrogenase Complex Subunit A) ELISA Kit

Catalog No. : ELK8423

Specification Price
48T $320
96T $458

Overview

Product name: Human SDHA(Succinate Dehydrogenase Complex Subunit A) ELISA Kit
Alternative Names: SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 0.132 ng/mL
Standard: 20 ng/mL
Range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 4.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit A(SDHA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Succinate Dehydrogenase Complex Subunit A(SDHA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Succinate Dehydrogenase Complex Subunit A(SDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Succinate Dehydrogenase Complex Subunit A(SDHA) in the samples is then determined by comparing the OD of the samples to the standard curve.