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Human ARL15(ADP Ribosylation Factor Like Protein 15) ELISA Kit

Catalog No. : ELK7390

Specification Price
48T $320
96T $458

Overview

Product name: Human ARL15(ADP Ribosylation Factor Like Protein 15) ELISA Kit
Alternative Names: ARFRP2; ADP-Ribosylation Factor Related Protein 2
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 4.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosylation Factor Like Protein 15(ARL15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADP Ribosylation Factor Like Protein 15(ARL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosylation Factor Like Protein 15(ARL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin ADP Ribosylation Factor Like Protein 15(ARL15) in the samples is then determined by comparing the OD of the samples to the standard curve.