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Human NDNF(Neuron Derived Neurotrophic Factor) ELISA Kit

Catalog No. : ELK7369

Specification Price
48T $320
96T $458

Overview

Product name: Human NDNF(Neuron Derived Neurotrophic Factor) ELISA Kit
Alternative Names: C4orf31
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 4.5h
Research Area: Cytokine;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuron Derived Neurotrophic Factor(NDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuron Derived Neurotrophic Factor(NDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuron Derived Neurotrophic Factor(NDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Neuron Derived Neurotrophic Factor(NDNF) in the samples is then determined by comparing the OD of the samples to the standard curve.