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Human bMSH(Beta-Melanocyte Stimulating Hormone) ELISA Kit

Catalog No. : ELK7352

Specification Price
48T $320
96T $458

Overview

Product name: Human bMSH(Beta-Melanocyte Stimulating Hormone) ELISA Kit
Alternative Names: β-MSH
Reacted Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 15.3 pg/mL
Standard: 3000 pg/mL
Range: 46.88-3000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2h
Research Area:
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Melanocyte Stimulating Hormone(bMSH) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Melanocyte Stimulating Hormone(bMSH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Melanocyte Stimulating Hormone(bMSH) in the samples is then determined by comparing the OD of the samples to the standard curve.