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Human RGS19(Regulator Of G Protein Signaling 19) ELISA Kit

Catalog No. : ELK7328

Specification Price
48T $320
96T $458

Overview

Product name: Human RGS19(Regulator Of G Protein Signaling 19) ELISA Kit
Alternative Names: GAIP; RGSGAIP; GNAI3IP; G-alpha-interacting protein
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 27 pg/mL
Standard: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 4.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 19(RGS19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 19(RGS19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 19(RGS19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Regulator Of G Protein Signaling 19(RGS19) in the samples is then determined by comparing the OD of the samples to the standard curve.