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Human KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit

Catalog No. : ELK7324

Specification Price
48T $320
96T $458

Overview

Product name: Human KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit
Alternative Names: INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 4.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1(KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Kelch Like ECH Associated Protein 1(KEAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.