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Human S100A15(S100 Calcium Binding Protein A15) ELISA Kit

Catalog No. : ELK7320

Specification Price
48T $320
96T $458

Overview

Product name: Human S100A15(S100 Calcium Binding Protein A15) ELISA Kit
Alternative Names: S100A7A; S100A7L1; S100A7f; S100 calcium-binding protein A7-like 1
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 4.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A15(S100A15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A15(S100A15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A15(S100A15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin S100 Calcium Binding Protein A15(S100A15) in the samples is then determined by comparing the OD of the samples to the standard curve.