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Human EGLN1(Egl Nine Homolog 1) ELISA Kit

Catalog No. : ELK7299

Specification Price
48T $320
96T $458

Overview

Product name: Human EGLN1(Egl Nine Homolog 1) ELISA Kit
Alternative Names: ECYT3; HIFPH2; PHD2; C1orf12; SM20; ZMYND6; HIF Prolyl Hydroxylase 2; Prolyl hydroxylase domain-containing protein 2; Hypoxia-inducible factor prolyl hydroxylase 2
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 13.5 pg/mL
Standard: 2000 pg/mL
Range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 4.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Egl Nine Homolog 1(EGLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Egl Nine Homolog 1(EGLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Egl Nine Homolog 1(EGLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Egl Nine Homolog 1(EGLN1) in the samples is then determined by comparing the OD of the samples to the standard curve.