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Human ECF(Eosinophil Chemotactic Factor) ELISA Kit

Catalog No. : ELK1009

Specification Price
48T $265
96T $376

Overview

Product name: Human ECF(Eosinophil Chemotactic Factor) ELISA Kit
Alternative Names: CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 6.4 pg/mL
Standard: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 4.5h
Research Area: Cytokine;Infection immunity;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor(ECF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor(ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eosinophil Chemotactic Factor(ECF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Eosinophil Chemotactic Factor(ECF) in the samples is then determined by comparing the OD of the samples to the standard curve.