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Human ANG(Angiogenin) ELISA Kit

Catalog No. : ELK1004

Specification Price
48T $265
96T $376

Overview

Product name: Human ANG(Angiogenin) ELISA Kit
Alternative Names: RNASE5; Ribonuclease,RNase A Family 5
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 13.5 pg/mL
Standard: 2000 pg/mL
Range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 4.5h
Research Area: Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenin(ANG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiogenin(ANG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiogenin(ANG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Angiogenin(ANG) in the samples is then determined by comparing the OD of the samples to the standard curve.