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Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

Catalog No. : ELK1000

Specification Price
48T $265
96T $376

Overview

Product name: Human ACE(Angiotensin I Converting Enzyme) ELISA Kit
Alternative Names: CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
Reacted Species: Human
Assay Type: Sandwich
Sensitivity: 0.16 ng/mL
Standard: 25 ng/mL
Range: 0.4-25 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 4.5h
Research Area: Enzyme & Kinase;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme(ACE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme(ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme(ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcitonin Angiotensin I Converting Enzyme(ACE) in the samples is then determined by comparing the OD of the samples to the standard curve.