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β-actin Rabbit pAb

Catalog No. : EA014

Specification Price
50µl $148
100µl $248

Overview

Product name: β-actin Rabbit pAb
Source: Rabbit
Applications: WB, IHC, IF
Species reactivity: Human,Mouse,Rat,Rabbit,Chicken,Monkey,Sheep,Xenopus
Recommended dilutions: WB 1:3,000 IHC 1:200 IF 1:200
Immunogen: Recombinant Protein
Specificity: Human
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Isotype: IgG
Concentration: 1mg/ml
Observed band: 43kDa
GeneID(Human): 60
Human Swiss-Prot No.: P60709
Cellular localization: Cytoplasm,Cytoskeleton
Alternative Names: actin antibody, b-actin antibody, beta-actin antibody
Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues. 
  • Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000.

  • IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200

  • IF analysis of Hela with EA014 diluted at 1:500.