β-actin Rabbit pAb
Catalog No. : EA014
|Product name：||β-actin Rabbit pAb|
|Applications：||WB, IHC, IF|
|Recommended dilutions：||WB 1:3,000 IHC 1:200 IF 1:200|
|Storage：||PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.|
|Human Swiss-Prot No.：||P60709|
|Alternative Names：||actin antibody, b-actin antibody, beta-actin antibody|
|Background：||β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.|
Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000.
IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200
IF analysis of Hela with EA014 diluted at 1:500.